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Fluorescence of spirochetes transformed with a GFP plasmid. B. afzelii #1192 transformed with in vitro methylated (a) and with unmethylated pBSV2_OspA_GFP (b). B. afzelii #1201 transformed with in vitro methylated (c) and with unmethylated pBSV2_OspA_GFP (d). B. garinii #1195 transformed with in vitro methylated pBSV2_OspA_GFP (e). B. garinii #1226 transformed with in vitro methylated pBSV2_OspA_GFP (f). All cultures were grown in modified BSK II medium at 34°C. All photos were taken using a Nikon Eclipse <t>TE300</t> Inverted Fluorescence Phase Contrast Microscope using a 40x objective.
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Fluorescence of spirochetes transformed with a GFP plasmid. B. afzelii #1192 transformed with in vitro methylated (a) and with unmethylated pBSV2_OspA_GFP (b). B. afzelii #1201 transformed with in vitro methylated (c) and with unmethylated pBSV2_OspA_GFP (d). B. garinii #1195 transformed with in vitro methylated pBSV2_OspA_GFP (e). B. garinii #1226 transformed with in vitro methylated pBSV2_OspA_GFP (f). All cultures were grown in modified BSK II medium at 34°C. All photos were taken using a Nikon Eclipse <t>TE300</t> Inverted Fluorescence Phase Contrast Microscope using a 40x objective.
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Fluorescence of spirochetes transformed with a GFP plasmid. B. afzelii #1192 transformed with in vitro methylated (a) and with unmethylated pBSV2_OspA_GFP (b). B. afzelii #1201 transformed with in vitro methylated (c) and with unmethylated pBSV2_OspA_GFP (d). B. garinii #1195 transformed with in vitro methylated pBSV2_OspA_GFP (e). B. garinii #1226 transformed with in vitro methylated pBSV2_OspA_GFP (f). All cultures were grown in modified BSK II medium at 34°C. All photos were taken using a Nikon Eclipse TE300 Inverted Fluorescence Phase Contrast Microscope using a 40x objective.

Journal: Current Research in Microbial Sciences

Article Title: Comparative analysis of Borrelia’s Defence mechanisms and their impact on genetic manipulation of low-passage isolates of Borrelia afzelii and Borrelia garinii

doi: 10.1016/j.crmicr.2025.100543

Figure Lengend Snippet: Fluorescence of spirochetes transformed with a GFP plasmid. B. afzelii #1192 transformed with in vitro methylated (a) and with unmethylated pBSV2_OspA_GFP (b). B. afzelii #1201 transformed with in vitro methylated (c) and with unmethylated pBSV2_OspA_GFP (d). B. garinii #1195 transformed with in vitro methylated pBSV2_OspA_GFP (e). B. garinii #1226 transformed with in vitro methylated pBSV2_OspA_GFP (f). All cultures were grown in modified BSK II medium at 34°C. All photos were taken using a Nikon Eclipse TE300 Inverted Fluorescence Phase Contrast Microscope using a 40x objective.

Article Snippet: All photos were taken using a Nikon Eclipse TE300 Inverted Fluorescence Phase Contrast Microscope using a 40x objective.

Techniques: Fluorescence, Transformation Assay, Plasmid Preparation, In Vitro, Methylation, Modification, Microscopy